Amusan, who is the 100mH world record holder, was a clear favourite ahead of the race, was shown a yellow after beating the gun for the first time but lined up again.
On the second time of the gun going off, Amusan did not make any mistake, sprinting to an easy win where she clocked a time of 12.89s.
The win means the Nigerian completed a hat-trick of African Games titles, having won the last two editions in Congo Brazzaville and Rabat.
The other Nigerian athlete in the race, Faith Osamuyi, just missed out on a podium finish after running 13.77s in fourth place.
